Experimentally elevated levels of testosterone at independence reduce fitness in a territorial bird.

Environmental conditions and individual strategies in early life may have a profound effect on fitness. A critical moment in the life of an organism occurs when an individual reaches independence and stops receiving benefits from its relatives. Understanding the consequences of individual strategies at the time of independence requires quantification of their fitness effects. We explored this period in the Red Grouse (Lagopus lagopus scoticus). In this system, testosterone and parasite (Trichostrongylus tenuis) levels are known to influence survival and reproduction, the two key components of individual fitness. We experimentally and simultaneously manipulated testosterone and parasites at three levels (high, intermediate, and control levels for both factors) in 195 young males in five populations using a factorial experimental design. We explored the effects of our treatments on fitness by monitoring reproduction and survival throughout the life of all males and estimating lambda(ind), a rate-sensitive index of fitness. Parasite challenges increased the number of worms with a time lag, as previously found. However, we did not find significant effects of parasite manipulations on fitness, possibly because parasite abundance did not increase to harmful levels. Our hormone manipulation was successful at increasing testosterone at three different levels. Such increases in hormone levels decreased overall fitness. This was caused by reduced offspring production in the first breeding attempt rather than by any effect of the treatment on bird survival. Our results highlight that investing in high testosterone levels at independence, a strategy that might enhance short-term recruitment probability in territorial species such as Red Grouse, has a fitness cost, and can influence the resolution of the trade-off between reproduction and survival later in life.

Condition- and parasite-dependent expression of a male-like trait in a female bird.

In many species, females display brightly coloured and elaborate traits similar to those that males use in intra- and inter-sexual selection processes. These female characters are sometimes related to fitness, and might function as secondary sexual characteristics that have evolved through sexual selection. Here, we used descriptive data from 674 females in 10 populations and an experimental removal of Trichostrongylus tenuis parasites in four populations, to examine the effects of season, age, condition, and parasites on the size of supra-orbital combs displayed by female red grouse Lagopus lagopus scoticus. We found that comb size (i) was greater during the breeding than the non-breeding season, (ii) was greater in adult than in young females, (iii) was positively correlated with body condition, and (iv) negatively correlated with parasite abundance. Experimentally, we showed that comb size increased proportionally to the number of worms removed after parasite dosing. Our findings provide a better understanding of proximate mechanisms behind the expression of a male-like trait in females, and we discuss its possible function as a female ornament.

Cervical myelopathy and congenital stenosis from hypoplasia of the atlas: report of three cases and literature review.

STUDY DESIGN: Case reports of patients with cervical myelopathy to hypoplasia of the atlas. OBJECTIVES: To report cases of cervical myelopathy due to congenital hypoplasia of the atlas and to review the literature. SUMMARY OF BACKGROUND DATA: Six previously documented cases of congenital hypoplasia of the atlas as a cause of cervical myelopathy are reported in the literature. METHODS: Three patient's clinical record and radiologic imaging studies as well as a thorough literature search are reported. Plain radiographs, computed tomography scans, magnetic resonance images, as well as somatosensory-evoked potential changes are displayed. RESULTS: Cervical myelopathy developed in three patients who were found to have congenital hypoplasia of the atlas. Laminectomy of C1 provided neurologic improvement in all three patients presented. CONCLUSION: Congenital hypoplasia of the atlas is a rare cause of cervical myelopathy. This report should broaden the radiographic differential diagnosis when seeking an explanation for the signs and symptoms of cervical myelopathy.

Cervical spine pedicle screws: a biomechanical comparison of two insertion techniques.

STUDY DESIGN: Biomechanical testing of the pullout strengths of pedicle screws placed by two different techniques in adult human cadaveric cervical spines. OBJECTIVES: To determine whether there is a significant difference in screw purchase of two commonly proposed methods of cervical pedicle screw insertion. SUMMARY OF BACKGROUND DATA: Wiring techniques remain the gold standard for posterior cervical fixation. However, absent or deficient posterior elements may dictate the use of alternative fixation techniques. Cervical pedicle screws have been shown to have significantly higher pullout strength than lateral mass screws. METHODS: Fifty fresh disarticulated human vertebrae (C3-C7) were evaluated with computed tomography for anatomic disease and pedicle morphometry. The right and left pedicles were randomly assigned to either a standard method or the Abumi insertion method. In the latter technique the cortex and cancellous bone of lateral mass are removed with a high-speed burr, which provides a direct view of the pedicle introitus. The pedicle is then probed and tapped and a 3.5-mm cortical screw inserted. Each screw was subjected to a uniaxial load to failure. RESULTS: There was no significant difference in the mean pullout resistance between the Abumi (696 N) and standard (636.5 N) insertion techniques (P = 0.41). There was no difference in pullout resistance between vertebral levels or within vertebral levels. Two (4%) minor pedicle wall violations were observed. CONCLUSION: In selected circumstances pedicle screw instrumentation of the cervical spine may be used to manage complex deformities and patterns of instability. Surgeons need not be concerned about reduced screw purchase when deciding between the Abumi method and its alternatives.

Cervical pedicle screws: comparative accuracy of two insertion techniques.

STUDY DESIGN: Independently assessed radiographic and anatomic comparison of device implantation methods. OBJECTIVES: To compare the relative accuracy of two techniques of inserting cervical pedicle screws. SUMMARY OF BACKGROUND DATA: In an attempt to define the anatomic risks of cervical pedicle screw insertion, image-guided stereotactic technology was shown to be superior to some other methods in vitro.- Meanwhile, in vivo experience with Abumi's technique of screw insertion has had few clinically relevant instances of screw malposition. There has been no direct comparison between current image-guided technology and Abumi's fluoroscopically assisted technique. METHODS: The pedicles (C3-C7) of human cadaveric cervical spines were instrumented with 3.5-mm screws with either of two techniques. Cortical integrity and potential neurovascular injury were independently assessed by computed tomographic (CT) scans and anatomic dissection. A cortical breach was considered "critical" if the screw encroached on any vital structure. If any part of the screw violated the cortex of the pedicle but no vital structure was at risk for injury, the breach was classified as "noncritical." RESULTS: In Group I (StealthStation; Sofamor-Danek, Memphis, TN), 82% of screws were placed in the pedicle, and 18% had a critical breach. In Group II (Abumi technique), 88% of screws were placed in the pedicle, and 12% had a critical breach. No statistically significant differences were demonstrated between each group (P = 0.59). Regarding pedicle dimensions and safety of insertion, a critical pedicle diameter of 4.5 mm was determined to be the size below which a critical breach was likely, but above which there was a significantly greater likelihood for safe screw placement. The most common structure injured in each group was the vertebral artery. CONCLUSIONS: The use of a computer-assisted image guidance system did not enhance safety or accuracy in placing pedicle screws compared with Abumi's technique. Both techniques have a noteworthy risk of injuring a critical structure if inserted into the pedicles with a diameter of less than 4.5 mm. Under laboratory conditions, pedicles with a diameter of more than 4.5 mm have a significantly greater likelihood of being safely instrumented by either technique. These data indicate that cervical pedicle screw placement is feasible, but it should be reserved for selected circumstances with clear indications and in the presence of suitable pedicle morphology.

Placement of pedicle screws in the human cadaveric cervical spine: comparative accuracy of three techniques.

STUDY DESIGN: This investigation was conducted in two parts. In the first part, a morphometric analysis of critical cervical pedicle dimensions were measured to create guidelines for cervical pedicle screw fixation based on posterior cervical topography. In the second part of the study, a human cadaver model was used to assess the accuracy and safety of transpedicular screw placement in the subaxial spine using three different surgical techniques: 1) using surface landmarks established in the first part of the study, 2) using supplemental visual and tactile cues provided by performing laminoforaminotomies, and 3) using a computer-assisted surgical guidance system. OBJECTIVE: To assess the accuracy of transpedicular screw placement in the cervical spine using three surgical techniques. SUMMARY OF BACKGROUND DATA: A three-column fixation device implanted to secure an unstable cervical spine can be a valuable tool with a biomechanical advantage in the spine surgeon's armamentarium. Despite this advantage, concerns over surgical neurovascular complications have surfaced. Cadaver-based morphometric measurements used to guide the surgeon in the placement of a pedicle screw show significant variability, raising legitimate concerns as to whether transpedicular fixation can be applied safely. METHODS: Precise measurements of 14 human cadaveric cervical spines were made by two independent examiners of pedicle dimensions, angulation, and offset relative to the lateral mass boundaries. On the basis of this analysis, guidelines for pedicle screw placement relative to posterior cervical topography were derived. In the second part of the study, 12 human cadaveric cervical spines were instrumented with 3.5-mm screws placed in the pedicles C3-C7 according to one of three techniques. Cortical integrity and neurovascular injury were then assessed by obtaining postoperative computed tomography scans (1-mm cuts) of each specimen. Cortical breaches were classified into critical or noncritical breaches. RESULTS: Linear measurements of pedicle dimensions had a wide range of values with only fair interobservercorrelation. Angular measurements showed similarangulation in the transverse plane (40 degrees ) at each level. With respect to the sagittal plane, both C3 and C4 pedicles were oriented superiorly relative to the axis of the lateral mass, whereas the C6 and C7 pedicles were oriented inferiorly. The dorsal entry point of the pedicle on the lateral mass defined by transverse and sagittal offset had similar mean values with wide ranges, although there often was excellent correlation between observers. There were no significant interlevel, right/left, or male/female differences noted with respect to offset. Using one of three techniques, 120 pedicles were instrumented. In group 1 (morphometric data): 12.5% of the screws were placed entirely within the pedicle; 21.9% had a noncritical breach; and 65. 5% had a critical breach. In group 2 (laminoforaminotomy), 45% of the screws were within the pedicle; 15.4% had a noncritical breach; and 39.6% had a critical breach. In group 3 (computer-assisted surgical guidance system), 76% of the screws were entirely within the pedicle; 13.4% had a noncritical breach; and 10.6% had a critical breach. Regardless of the technique used, the vertebral artery was the structure most likely to be injured. CONCLUSIONS: On the basis of the morphometric data, guidelines for cervical spine pedicle screw placement at each subaxial level were derived. Although a statistical analysis of cadaveric morphometric data obtained from the cervical spine could provide guidelines for transpedicular screw placement based on topographic landmarks, sufficient variation exists to preclude safe instrumentation without additional anatomic data. Insufficient correlation between different surgeons' assessments of surface landmarks attests to the inadequacy of screw insertion techniques in the cervical spine based on such specific topographic guide

Osteoinductive bone graft substitutes.

This review will summarize the major efforts currently underway to develop osteoinductive bone graft substitutes for minimally invasive spine fusions. The primary categories of substitute include purified bone growth factors, recombinant bone growth factors, and growth factors delivered by gene therapy approaches. Clinical trials are underway for the purified and recombinant bone growth factors and pre-clinical studies have yielded promising results for a variety of gene therapy techniques for generating bone.

Osteoinductive bone graft substitutes for spinal fusion: a basic science summary.

An understanding of precise biologic mechanisms at work during spinal fusion healing is just beginning to evolve. Current molecular biology research has shown this process to be multifactorial and extremely complex. With the advent of improved animal models to study the biology of spinal fusion, essential information regarding the basic science behind arthrodesis has advanced knowledge of this process. Moreover, with advances in local gene therapy as well as osteoinductive proteins and osteoinductive carrier matrices, the orthopedic surgeon will soon enter into a new era of biologic manipulation for fusion.

The efficacy of using an image-guided Kerrison punch in performing an anterior cervical foraminotomy. An anatomic analysis.

STUDY DESIGN: This study comprised two parts: first, a feasibility study to determine the efficacy of using an image-guided Kerrison punch while performing a foraminotomy during an anterior cervical decompression and, second, an anatomic analysis using vector measurement to determine the distance from the entrance of the neuroforamen to the medial margin of the vertebral artery in the subaxial cervical spine. OBJECTIVE: To assess the feasibility of using an image-guided Kerrison punch when performing an anterior foraminotomy and to obtain data regarding the distance from the vertebral artery to the entrance of the neuroforamen. SUMMARY OF BACKGROUND DATA: The documented incidence of catastrophic iatrogenic vertebral artery injury in anterior cervical decompression is low. The use of a real-time image-guidance surgical system should reduce the risk of this complication. METHODS: Twelve cadaveric cervical spines were harvested. Standard anterior cervical discectomies with bilateral foraminotomies were performed in the subaxial cervical spine using an image-guided Kerrison. Surgically significant morphometric data were measured using a computer-assisted image-guided surgical system. RESULTS: Successful navigation into all neuroforamina in the subaxial cervical spine was attained using the image-guided Kerrison punch. The vector measurement from the neuroforamen to the vertebral artery averaged 5.8 +/- 1.2 mm at C3-C4, 6.5 +/- 1.6 mm at C4-C5, 7.9 +/- 1.4 mm at C5-C6, and 9.1 +/- 1.8 mm at C6-C7. Statistically significant differences (P < 0.05) were found between all cervical levels except C3-C4 and C4-C5. CONCLUSION: An image-guided Kerrison punch may be used successfully when performing cervical foraminotomies during an anterior cervical discectomy, thus eliminating the risk of potential vertebral artery injury. These data confirm previous findings by other authors. Knowledge of these data may aid the spine surgeon in performing a foraminotomy during anterior cervical decompression.

Transpedicle screw fixation of the cervical spine.

The use of posterior cervical spine fixation has become increasingly popular in recent years. Dissatisfaction with lateral mass fixation, especially at the cervicothoracic junction, has led spine surgeons to use cervical pedicle screw fixation for reconstruction in numerous cervical spine disorders. The biomechanical advantage of a three-column fixation device implanted to secure an unstable cervical spine has proven to be a valuable tool in the spine surgeon's armamentarium. Successful placement of a pedicle screw in the cervical spine requires a sufficient three-dimensional understanding of pedicle morphology to allow accurate identification of the ideal screw axis. Variability in cadaveric based morphometric measurements used to guide the surgeon in the placement of a pedicle screw has raised legitimate concerns as to whether transpedicle fixation can be applied without significant neurovascular complications. The emergence of computer assisted image guidance systems may be implemented in the operative protocol to improve the accurate placement of a pedicle screw. The indications for placement of a pedicle screw in the cervical spine are beginning to evolve. Only surgeons experienced in transpedicle screw fixation and surgery of the cervical spine should perform this method of instrumentation.

Bilateral peroneal nerve palsy secondary to a knee board: report of two cases.

We report on two patients who developed bilateral peroneal nerve palsy after using a knee board behind a water ski boat. This device causes the rider's knees to be in a hyperflexed position secured with a strap across the thighs. Treatment for this compressive neuropathy is conservative. Recreational users may wish to limit the duration and frequency of participation in this sport, thus decreasing the predisposition to prolonged nerve compression. In addition, manufacturers may consider making fundamental design changes such as padding the nylon straps or outrigger devices that contact the proximal lateral tibia.

Surgical anatomy of the vertebral arteries.

STUDY DESIGN: This study compared direct measurements of the distances between the vertebral arteries in the cervical spines of human cadaver specimens with data obtained from axial computed tomography images of these specimens. OBJECTIVE: To determine whether the information obtained from a computed tomography scan can be used reliably to predict the true anatomic location of the vertebral arteries and, in so doing, provide accurate guidelines for the lateral extent of anterior cervical decompressive procedures. SUMMARY OF BACKGROUND DATA: Iatrogenic vertebral artery injury during anterior cervical surgery is uncommon, potentially catastrophic, and avoidable. METHODS: The means and standard deviation of measurements of the location of the cervical segment of the vertebral arteries obtained with high-precision, digital calipers by direct gross anatomic dissection of 16 adult (eight male, eight female) cadaver specimens were recorded. These measurements were compared with computed tomography scan data obtained on the same specimens. RESULTS: The mean distances between the vertebral arteries progressively increased from C3 to C6. Computed tomography scan measurements of the distance between the cervical foramina transversaria were consistently smaller than direct measurements of the gross specimens. At C6, the computed tomography scan data were significantly less than the gross anatomic data. CONCLUSIONS: According to these data, computed tomography scan measurements may be used safely and accurately to plan the lateral extent of anterior cervical decompressive surgical procedures. Although the data obtained from the gross anatomic dissections may serve as guidelines to assist the surgeon, the authors recommend a careful review of the preoperative computed tomography scan on an individual case-by-case basis as the safest method to plan for anterior cervical surgery.

Acute toxic methemoglobinemia following dental analgesia.

This communication reports a case of acute toxic methemoglobinemia associated with hypoxia and general motor seizures following dental anesthesia using nitrous oxide and prilocaine hydrochloride. The dosage of prilocaine hydrochloride used was 5.6% of the recognized toxic dosage associated with clinical methemoglobinemia. The patient had no demonstrable congenital tendency toward methemoglobinemia, suggesting this case may represent a previously unreported and potentially fatal reaction to prilocaine hydrochloride. The etiology of the methemoglobin production, the diagnosis of the syndrome, and the emergency treatment are discussed.